Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells
Figure 6
Biological effects of rATB on PBMCs. Relative concentration of TNF-α (a) and IL-6 (b) in culture of PBMCs (5 × 105/mL) treated with rATB (0.8–5000 ng/mL). Data from three individual donors are shown. Measurements were performed in triplicate. Means ± s.d. are shown. (c) Relative concentration of TNF-α and IL-6 in culture of PBMCs (5 × 105/mL), preincubated with Gal (50 mM), Man (50 mM), or vehicle buffer before rATB (500 ng/mL) treatment. Values shown are averages ± s.d. of one experiment assayed in triplicate. * versus vehicle control. (d) Viability of PBMCs treated with 100–5000 ng/mL rATB. The cell viability with vehicle buffer treatment is set as 100%. Values are expressed as the mean of three replicates ± s.d.
