Leea indica Ethyl Acetate Fraction Induces Growth-Inhibitory Effect in Various Cancer Cell Lines and Apoptosis in Ca Ski Human Cervical Epidermoid Carcinoma Cells
Effect of LIEAF on the change of mitochondrial membrane potential in Ca Ski cells. Ca Ski cells were incubated in the absence (control) or with varying concentrations (100, 500, and 1000 μg/mL) of LIEAF for 24 hours. In another experiment, Ca Ski cells were incubated in the absence (control) or presence of 500 μg/mL of LIEAF for 24, 48, and 72 hours. The cells were then stained with JC-1 and analyzed with flow cytometer. (a) Flow cytometric fluorescence patterns analysis of JC-1 staining. (b) Bar charts showed the quantitative presentation of the data as red (585 nm)/green (530 nm) fluorescence, expressed as percentage of control, indicated ratio of high/low MMP. Data presented are representative of means ± S.E. calculated from three individual experiments. The asterisk (*) represented significantly different value from control (*). (c) Ca ski cells were treated without (control) or with indicated concentration of LIEAF for 24 hours. The cells were stained with JC-1 and analyzed by fluorescent microscope. Red fluorescence represented J-aggregate form and green fluorescence represented J-monomer form. Magnification is 400x.