Aqueous Extract of Shi-Liu-Wei-Liu-Qi-Yin Induces G2/M Phase Arrest and Apoptosis in Human Bladder Carcinoma Cells via Fas and Mitochondrial Pathway
Figure 6
SLWLQY induced apoptotic cell death in TSGH-8301 cells. Culture cells were treated without (control) or with SLWLQY at the doses of 1, 3 and 5 mg ml−1 for 24 h. These proteins were detected by anti-caspases-3, caspase-8, caspase-9 and cytochrome c antibodies. The expression of cytochrome c in the cytosol and the mitochondria was assayed by immunoblotting (a) and anti-Fas/FasL antibody (b). (c) Western blot analysis of Fas protein expression and caspase-3 cleavage in cell lysates transfected with Fas siRNA and nonspecific siRNA as well as exposed to SLWLQY for 24 h. β-actin and tubulin were the loading control. Arrows indicated the cleavage fragment of caspases. Results were averaged from triplicate experiments and expressed in mean ± SD. *P < .05, **P < .01.
