Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation
Figure 3
Expression of macrophage differentiation markers in F3-induced THP-1 cells were examined by Q-PCR and western blotting. (a) Gene expressions of classical markers of macrophage differentiation, CD11b, CD14 and CD68, were determined by Q-PCR. (b) Other macrophage differentiation markers, MPO and MMP9, were also determined by Q-PCR and western blotting. mRNA expression of these genes was normalized to RNA content for each sample by using β-actin as internal controls; β-actin was also used as internal loading controls in western blotting.
