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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 378907, 9 pages
http://dx.doi.org/10.1155/2011/378907
Research Article

Neuron Regeneration and Proliferation Effects of Danshen and Tanshinone IIA

1Department of Dermatology, Taichung Veterans General Hospital, Taichung 40705, Taiwan
2Institute of Medicine, Chung Shan Medical University, Taichung 40705, Taiwan
3Graduate Institute of Chinese Medical Science, China Medical University, Taichung 404, Taiwan
4Department of Medical Imaging and Radiological Science, Central Taiwan University of Science and Technology, Taichung 40705, Taiwan
5Graduate Institute of Chinese Pharmaceutical Sciences, China Medical University, Taichung 40705, Taiwan
6Laboratory of Exercise Biochemistry, Taipei Physical Education College, Taipei 105, Taiwan
7Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 40705, Taiwan
8Department of Pediatrics, Medical Research and Medical Genetics, China Medical University, Taichung 40705, Taiwan
9School of Medicine, Chung Shan Medical University, Taichung 402, Taiwan
10Department of Surgery, Chung Shan Medical University Hospital, Taichung 402, Taiwan
11Graduate Institute of Basic Medical Science, China Medical University, 40705 Taichung, Taiwan
12Department of Health and Nutrition Biotechnology, Asia University, Taichung 413, Taiwan
13Orthopaedic Department, Taichung, Armed Forces General Hospital, Taichung 41152, Taiwan
14Department of Materials and Science and Engineering, National Chung Hsing University, Taichung 402, Taiwan

Received 10 November 2009; Revised 24 August 2010; Accepted 10 October 2010

Copyright © 2011 Jui-Lung Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study evaluates the proliferative effects of danshen and its monomer extract, tanshinone IIA, on Schwann cell proliferation. A piece of silicone rubber was guided across a 15-mm gap in the sciatic nerve of a rat. This nerve gap was then filled with different concentrations of danshen (0–100 mg/mL). The results showed that danshen increased the expressions of uPA, cyclin D1, E and ERK, JNK, and P38 MAP kinases via the FGF-2 signaling pathway in a dose-dependent manner. RSC96, Schwann cells were also administered with danshen (0, 20, 40, 60, 80, and 100 μg/mL) and tanshinone IIA (0, 2, 4, 6, 8, and 10 μg/mL). In lower concentrations, danshen and tanshinone IIA exhibited an apparent effect on Schwann cells. Similar effects were also demonstrated in the FGF-2-uPA regulating cascade and cell cycle proliferative protein results. Schwann cell migration was elevated as well. We used MAPK-signaling chemical inhibitors and identified the proliferative effects of danshen and tanshinone IIA as MAPK-signaling dependent. The results from the in vitro systems indicate that danshen and tanshinone IIA can be used to induce Schwann cell proliferation, and in vivo results potentially suggest that danshen and tanshinone IIA might enhance neuron regeneration.