Neuron Regeneration and Proliferation Effects of Danshen and Tanshinone IIA
Figure 4
The proliferative effect of danshen and tanshinone IIA on RSC96 cell viability has ERK-, JNK- and P38-signaling dependent. The RSC96 cells were treated with various doses of danshen and tanshinone IIA. The treatments and procedures are the same as those described for Figure 3. The MAPK-signaling activities were measured using western blotting (a). The RSC96 cells were treated with danshen and tanshinone IIA at concentrations of 40 μg/mL and 4 μg/mL, respectively, and MAPK signaling inhibitors as indicated. Cell viability was examined using MTT assay (b); the cell proliferation was analyzed using western blot analysis of PCNA (c). α-tubulin was used as a loading control. U0126, SB203508, and SP600125 are inhibitors of MEK, P38, and JNK, respectively. ## and ### denote significant differences between experimental and control values with , and . *, **, and *** denote significant differences between danshen and tanshinone IIA values with , , and .
