380923.fig.004a
(a)
380923.fig.004b
(b)
Figure 4: Western blot analysis of cell cycle regulatory protein levels and apoptosis proteins in TCCSUP cells treated with Florin extracts. (a) Cdc25C, Cdc-2, pCdc-2, cyclin-B1, and Bcl-2 proteins were analyzed after cells were exposed to Florin extracts. Cells were treated with 25 μg/mL of Florin extracts for 6, 12, 24, and 36 h. Proteins (50 μg) from each sample was resolved on 12% SDS-PAGE and Western blot was performed. GAPDH was used as a control. The thick and thin bars indicate the locations of the fast (dephosphorylated) and slow (phosphorylated) migrating forms of cyclin B1, P-Cdc2, or Cdc2, respectively. (b) Expression of Bcl-2 and Bax proteins in TCCSUP cells was analyzed. Cells were treated with 6, 12, and 25 μg/mL of Florin extracts for 24 h. Proteins (50 μg) from each sample were resolved on 12% SDS-PAGE and Western blot was performed. GAPDH was used as a control. The two slow migrating forms of Bcl2 may be the hyperphosphorylated forms of Bcl2.