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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 393752, 6 pages
http://dx.doi.org/10.1155/2011/393752
Research Article

Description of a Sulfitobacter Strain and Its Extracellular Cyclodipeptides

1Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
2Department of Clinical Laboratory, First Affiliated Hospital of Yangtze University, No. 8 Hangkong Road, Hubei Jingzhou 434100, China

Received 14 January 2011; Accepted 16 May 2011

Copyright © 2011 Cong Long et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A marine bacterium M44 was separated from 30 m deep seawater in the East China Sea (26° 28.3 N 122° 29.0 E) in 2006. 16S rDNA gene sequence comparison showed that the strain M44 was a member of the genus Sulfitobacter and highly similar to KMM 3554T. A series of experiments demonstrated that this strain M44 had many distinctive characteristics: its cells were gram-negative and mesophilic; its colonies were slightly yellowish, round, convex, and smooth; and it could grow at 10–28°C, pH 6.0–10.0, and in the presence of 0–12.5% (w/v) NaCl; the optimum growth conditions were 25°C and pH 7.0, and the optimum Na+ concentration was 2.5%. In addition, strain M44 contained 18 : 1 ω7c, 11 methyl 18 : 1 ω7c and 16 : 0 fatty acids as major fatty acids, and the genomic DNA G+C content was 58.04 mol%. According to our results of the secondary metabolites, six cyclodipeptides were isolated from the strain M44, which were Cyclo (Val-Leu), Cyclo (Phe-Val), Cyclo (Phe-Leu), Cyclo (Leu-Ile), Cyclo (Phe-Ile), and Cyclo (Trp-Pro). It is the first study of secondary metabolites isolated from this genus.