In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid-Rich Fraction from Typhonium flagelliforme Tuber
SEM micrographs of surface ultrastructural characteristics of CEMss cells treated with DCM/F7 in a time-dependent manner (24 and 48 h) and cultured in RPMI 1640 media maintained at 37°C and 5% CO2. (a) The characteristic of untreated CEMss cells surface showing the restoration of a typical morphological feature of a cancer cell such as smooth surface (as shown in white arrow). (b) and (c) DCM/F7 treated CEMss cells (24 and 48; IC50: 3 μg/ml) showed distinct morphological changes corresponding to typical apoptosis, including cell shrinkage and membrane blebbing and separated apoptotic bodies (magnification 4000x). BL: Blebbing; AB: Apoptotic body.