In Vitro Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid-Rich Fraction from Typhonium flagelliforme Tuber
Electromicrograph of untreated human T4 lymphoblastoid leukemia cells (CEMss) demonstrates the normal structure of CEMss cancer cell. The nucleus (N) contains evenly distributed chromatin and a large nucleolus (Nu), mitochondria (M) are large and numerous, rough endoplasmic reticulum (RER), ribosome (R) and the double membrane nuclear envelop (arrow) are also seen (×1000). In the treated cell nuclear chromatin marginisation (b-cm) (×1500), chromatin condensation (c-cc) (×1500) formation of apoptosis micronuclei (b-mn) characterize the apoptosis cell. The vacuolization (v) is also seen. Mitochondria of the control cell (a-m) shows more or less parallel cristae and of a treated cell (c-m) show vacuolization and loss of cristae. Cells were cultured in RPMI 1640 (25 ml flask) media maintained at 37°C and 5% CO2.