Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata: Holothuroidea)

Figure 4

Analysis of fractions of gel filtration chromatography of Cuvierian tubule extract. (a) 15% SDS PAGE. CT: extract of Cuvierian tubules in 4 M urea, 0.5 M Tris-HCl buffer pH 7.5. AP: adhesive protein obtained by electroelution from Cuvierian tubule extract (positive control). 6–11: fractions with highest concentration of protein according to Bradford assay. M, Molecular mass markers. Arrowhead: adhesive protein (18 kDa). (b) Adhesive activity, given in arbitrary units ± SD (𝑛=3), of fractions 6–11 compared to control (0.5 M urea buffer). Numbers to the left indicate molecular masses of marker proteins in kDa. Level of significance: *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001.

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