Anticancer Potentials of Root Extract of Polygala senega and Its PLGA Nanoparticles-Encapsulated Form
Figure 5
(a) EEPS and NEEPS induced apoptosis in A549 cells by TUNEL assay. Ten thousand events were collected and analysed for each sample. (i) A549 cells cultured in DMEM with 10% FBS for 24 hours. (ii)-(iii) Cells treated with two doses of EEPS (100 and 200 g), and (iv)-(v) Cells treated with two doses of NEEPS (100 and 200 g). The viable cells are located in the lower left corner (negative for both FITC and PI). Early apoptotic cells and late apoptotic cells are in the lower right corner and upper right corner (double positive), respectively. Necrotic cells lacking a cell membrane structure are in the upper left corner (PI positive). The numbers in the figure represent the percentage of the total cell population. (b) The histogram represents percentages of cell population (viable, early apoptotic, late apoptotic, and necrotic cells) in different series of TUNEL assay. Value represents mean SE (). Significant differences between EEPS and NEEPS are indicated by *() and ***().
