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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 519517, 7 pages
http://dx.doi.org/10.1093/ecam/nen053
Original Article

An Antioxidant Phytotherapy to Rescue Neuronal Oxidative Stress

1Department of Chinese Medicinal Prescription, China Pharmaceutical University, 639 Longmian Avenue, Jiangning University City, Nanjing, Jiangsu 211198, China
2Department of Pharmacognosy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China
3Department of Physiology, China Pharmaceutical University, Nanjing, Jiangsu 211198, China
4National Engineering Research Center for Traditional Chinese Medicine, Shanghai 201203, China

Received 16 January 2008; Accepted 15 July 2008

Copyright © 2011 Zhihong Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Oxidative stress is involved in the pathogenesis of ischemic neuronal injury. A Chinese herbal formula composed of Poria cocos (Chinese name: Fu Ling), Atractylodes macrocephala (Chinese name: Bai Zhu) and Angelica sinensis (Chinese names: Danggui, Dong quai, Donggui; Korean name: Danggwi) (FBD), has been proved to be beneficial in the treatment of cerebral ischemia/reperfusion (I/R).This study was carried out to evaluate the protective effect of FBD against neuronal oxidative stress in vivo and in vitro. Rat I/R were established by middle cerebral artery occlusion (MCAO) for 1 h, followed by 24 h reperfusion. MCAO led to significant depletion in superoxide dismutase and glutathione and rise in lipid peroxidation (LPO) and nitric oxide in brain. The neurological deficit and brain infarction were also significantly elevated by MCAO as compared with sham-operated group. All the brain oxidative stress and damage were significantly attenuated by 7 days pretreatment with the aqueous extract of FBD (250 mg kg−1, p.o.). Moreover, cerebrospinal fluid sampled from FBD-pretreated rats protected PC12 cells against oxidative insult induced by 0.2 mM hydrogen peroxide, in a concentration and time-dependent manner (IC50 10.6%, ET50 1.2 h). However, aqueous extract of FBD just slightly scavenged superoxide anion radical generated in xanthine–xanthine oxidase system (IC50 2.4 mg ml−1) and hydroxyl radical generated in Fenton reaction system (IC50 3.6 mg ml−1). In conclusion, FBD was a distinct antioxidant phytotherapy to rescue neuronal oxidative stress, through blocking LPO, restoring endogenous antioxidant system, but not scavenging free radicals.