Oral Treatment with Extract of Agaricus blazei Murill Enhanced Th1 Response through Intestinal Epithelial Cells and Suppressed OVA-Sensitized Allergy in Mice
Figure 8
Effect of anti-FIII-2-b antibody on TNF- production induced by ABME in a coculture system. Anti-FIII-2-b antibody or isotype IgG1 (25 ml/well) were incubated with ABME for 2 h and centrifuged at 7000rpm for 5 minutes. The supernatant was added to the apical compartment of the Caco-2/RAW264.7 co-culture system for 3 h. After the incubation, the supernatant from the basolateral side was collected, and TNF- content was measured using a killing assay. Values represent the means ± S.E. (). and , significantly different from the values for FIII-2-b and ABME treatment, respectively.
