Gastroprotective Effect of Rubia cordifolia Linn. on Aspirin Plus Pylorus-Ligated Ulcer

Table 2

Effect of R. cordifolia on antioxidant parameters in stomach of aspirin + pylorus-ligated rats.

1

2

3

4

5

6

7

8

9

10

11

12

Group

Normal

Control

Standard

ME 100

ME 200

ME 400

CF 50

CF 100

CF 200

RF 50

RF 100

RF 200

SOD (Units/gm of wet tissue)

204.4 ± 3.61

89.4±2.94^a

125.2 ± 2.38***

104.48±2.09^NS

105.24±1.48^NS

112.36 ± 2.94***

110.84 ± 3.72**

132.92 ± 2.65***

172.06 ± 7.22***

98.54±3.57^NS

102.68±1.76^NS

106.14 ± 2.2*

CAT (Units/gm of wet tissue)

45.97 ± 2.12

13.04±1.24^a

40.35 ± 1.25***

18.96±1.72^NS

23.73 ± 1.26**

26.3 ± 1.47***

24.3 ± 1.86**

29.73 ± 2.05***

34.4 ± 2.02***

17.18±1.62^NS

18.84±1.98^NS

20.35±1.95^NS

GSH (nmols/gm of wet tissue)

218.4 ± 6.43

94.9±3.42^a

142.9 ± 6.72***

122.22 ± 4.4*

127.28 ± 4.72**

150.52 ± 3.84***

123.96 ± 4.97**

155.4 ± 4.52***

176.42 ± 4.59***

108.28±4.8^NS

111.66±5.7^NS

120.4 ± 4.81*

LPO (nmols of MDA/gm of wet tissue)

139.68 ± 4.27

258.48±3.72^a

235.64 ± 6.19*

236.8±6.85^NS

229.24 ± 5.38**

183.46 ± 3.76***

199.66 ± 4.74***

177.76 ± 3.95***

155.94 ± 2.73***

244.94±2.75^NS

237.76±3.28^NS

230.1 ± 3.9**

𝑁=6 normal group= vehicle Na CMC 1% (10 ml/ kg, p.o.).
Values are expressed as mean ± SEM. Control group was compared with normal group. Test and standard groups were compared with control group. Statistical comparison was performed using analysis of variance (ANOVA) followed by Turkey’s test. ^a𝑃<.001, when compared with normal group. *𝑃<.05; **𝑃<.01; ***𝑃<.001; NS: nonsignificant, when compared with control groups.
ME100, 200, and 400—methanolic Extract of Rubia cordifolia 100, 200, and 400 mg/kg, p.o., respectively, CF50,100, and 200—chloroform fraction of Rubia cordifolia 50, 100, and 200 mg/kg, p.o., respectively, RF50,100, and 200—residual fraction of Rubia cordifolia 50, 100, and 200 mg/kg, p.o., respectively.