Original Article

Insulin-Mimetic Action of Rhoifolin and Cosmosiin Isolated from Citrus grandis (L.) Osbeck Leaves: Enhanced Adiponectin Secretion and Insulin Receptor Phosphorylation in 3T3-L1 Cells

Figure 5

Rhoifolin and cosmosiin stimulated GLUT4 translocation in the 3T3-L1 adipocytes. Cells were starved for 3 h in serum-free medium, then insulin, or different concentrations of rhoifolin and cosmosiin added for 30 min. The subsequent GLUT4 translocation was visualized by fluorescent immunostaining. GLUT4 (red) immunoreactivity was observed near the nucleus (blue) or distributed evenly on the plasma membrane.
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