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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 704721, 8 pages
Original Article

In Vitro Activity of 2-methoxy-1,4-naphthoquinone and Stigmasta-7,22-diene-3β-ol from Impatiens balsamina L. against Multiple Antibiotic-Resistant Helicobacter pylori

1Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
2Division of Gastroenterology, Department of Internal Medicine, Kaoshing Medical University, Kaohsiung, Taiwan
3Graduate Institute of Healthcare Administration, College of Health Sciences, Kaoshing Medical University, Kaohsiung, Taiwan
4Faculty of Medicinal and Applied Chemistry, Kaoshing Medical University, Kaohsiung, Taiwan
5Department of Chemistry, National Chung Hsing University, Taichung, Taiwan

Received 22 April 2009; Accepted 30 August 2009

Copyright © 2011 Yuan-Chuen Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Infection with Helicobacter pylori is strongly associated with gastric cancer and gastric adenocarcinoma. WHO classified H. pylori as a group 1 carcinogen in 1994. Impatiens balsamina L. has been used as indigenous medicine in Asia for the treatment of rheumatism, fractures and fingernail inflammation. In this study, we isolated anti-H. pylori compounds from this plant and investigated their anti- and bactericidal activity. Compounds of 2-methoxy-1,4-naphthoquinone (MeONQ) and stigmasta-7,22-diene-3β-ol (spinasterol) were isolated from the pods and roots/stems/leaves of I. balsamina L., respectively. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) for MeONQ were in the ranges of 0.156–0.625 and 0.313–0.625 μg mL−1, respectively, and in the ranges of 20–80 μg mL−1 both of MICs and MBCs for spinasterol against antibiotic (clarithromycin, metronidazole and levofloxacin) resistant H. pylori. Notably, the activity of MeONQ was equivalent to that of amoxicillin (AMX). The bactericidal H. pylori action of MeONQ was dose-dependent. Furthermore, the activity of MeONQ was not influenced by the environmental pH values (4–8) and demonstrated good thermal (121°C for 15 min) stability. MeONQ abounds in the I. balsamina L. pod at the level of 4.39% (w/w db). In conclusion, MeONQ exhibits strong potential to be developed as a candidate agent for the eradication of H. pylori infection.