Antiosteoporotic Activity of Dioscorea alata L. cv. Phyto through Driving Mesenchymal Stem Cells Differentiation for Bone Formation
Figure 5
Dispo85E stimulates osteoblastogenesis in bone-marrow-derived cells isolated from OVX mice. C57BL/6 mice were sham or OVX. The OVX mice were fed 0 mg/kg/day (vehicle control), 40 mg/kg/day, 200 mg/kg/day, or 1000 mg/kg/day Dispo85E for 42 days. (a) Expression of the mRNA for different bone regulatory factors and osteoblastic markers in OVX mouse bone-marrow-derived cells was evaluated by RT-PCR. (b) Cellular ALP activity and (c) nodule formation assay were performed as described in Section 2. Cells were stained with Alizarin red S for osteoblasts, and (d) the mineralized area was quantified by extracting alizarin red S staining with 10% CPC and measuring the OD of the extract at 550 nm. **Indicates significant difference from OVX mice at ; *Indicates significance at . n =10-11 for each group.
