Low-Frequency Electroacupuncture Improves Insulin Sensitivity in Obese Diabetic Mice through Activation of SIRT1/PGC-1 in Skeletal Muscle
Figure 3
Effect of electroacupuncture on SITR1, PGC-1α, NRF1, and ACOX gene expressions in skeletal muscles of nondiabetic db/m and diabetic db/db mice. (a) Electroacupuncture had no significant effect on SIRT1 mRNA levels in db/m and db/db mice. (b) Electroacupuncture upregulated PGC-1α mRNA levels in both db/m and db/db mice. (c) Electroacupuncture increased NRF1 mRNA levels in db/db mice. (d) Electroacupuncture increased ACOX mRNA levels in db/m and db/db mice. Quadriceps muscles of the mice were collected for mRNA expression, which was estimated using quantitative real-time RT-PCR and normalized to the expression of GAPDH. Graph shows the percentage of mRNA relative to GAPDH in each group. Each value represents the mean ± SE of four mice. versus db/m, versus db/db, and versus db/m.
