KIOM-79, an Inhibitor of AGEs–Protein Cross-linking, Prevents Progression of Nephropathy in Zucker Diabetic Fatty Rats
Figure 4
Effect of KIOM-79 on collagen accumulation and on expression of fibronectin and TGF-β1 in the renal cortex of ZF rats. (a) Masson's trichrome stain shows collagen accumulation in the renal cortex from normal lean rat, ZF rat, ZF rat treated with AG (50 mg/kg), and ZF rat treated with KIOM-79 (50 mg/kg). ×400. (b) Morphometric analysis of Masson's trichrome-positive areas in renal cortex in each group. The renal cortex from rats was immunoblotted using specific antibodies for fibronectin (d) and TGF-β1 (g). Total RNA was isolated and collagen III (c), fibronectin (f) and TGF-β1 (h) expression were measured by RT-PCR. All data are expressed as mean ± SD (, resp.).*** versus ZL group; ###; ##; #, versus ZF group, respectively.
