Role of Bone Morphogenetic Proteins-7 (BMP-7) in the Renal Improvement Effect of DangGui (Angelica sinensis) in Type-1 Diabetic Rats

Figure 4

Effect of DangGui on the BMP-7 level in high-glucose-treated RMCs. RMCs were cultured in normal glucose (5 mmol/L; Con) or high-glucose (30 mmol/L; HG) medium in the presence of 200 μg/mL DangGui (DG) for 24 h incubation. Western blot analysis of BMP-7 expression in RMCs. Upper picture shows the protein level of BMP-7 or β-actin in RMCs. The conditional treatments on RMCs are described in the Materials and methods section. Quantification of protein level using BMP-7/β-actin expressed as mean ± SE (n = 4 per group) in each column is indicated in the lower panel. *P < 0.05 compared to the value of control medium.