Role of Bone Morphogenetic Proteins-7 (BMP-7) in the Renal Improvement Effect of DangGui (Angelica sinensis) in Type-1 Diabetic Rats

Figure 5

Effect of DangGui on BMP-7 protein in RMCs-treated BMP-7 siRNA. RMCs were cultured in scramble siRNA (Con) or BMP-7 siRNA (siBMP-7) transfection conditional medium for 12 h. RMCs were cultured in normal glucose (5 mmol/L) medium in the presence of 200 μg/mL DangGui (DG) after being transfected with scramble siRNA (Con+DG) or BMP-7 siRNA (siBMP-7+DG) for further 24 h incubation. Western blot analysis of BMP-7 expression in RMCs. Upper picture shows the protein level of BMP-7 or β-actin in RMCs. The conditional treatments on RMCs are described in the Materials section. Quantification of protein level using BMP-7/β-actin expressed as mean ± SE (n = 4 per group) in each column is indicated in the lower panel. *P < 0.05 compared to the value after DangGui treatment.