Neuroprotection by the Traditional Chinese Medicine, Tao-Hong-Si-Wu-Tang, against Middle Cerebral Artery Occlusion-Induced Cerebral Ischemia in Rats
Effects of Tao-Hong-Si-Wu-Tang (THSWT) on the expressions of (a) hypoxia-inducible factor (HIF-1 and (b) inducible nitric oxide synthase (iNOS) in cerebral homogenates 24 h after middle cerebral artery occlusion-(MCAO-) reperfusion injury in rats. Fresh brains from sham-operated (lane 1), solvent-treated (lane 2), and THSWT- (0.7 g ) treated (lane 3) rats were removed and sectioned coronally into four sequential parts from the frontal lobe to the occipital lobe. The third of four sequential parts of the ischemic-injured hemisphere was separately collected, homogenized, and centrifuged. The supernatant (50 g protein) was then subjected to SDS-PAGE, and transferred onto membranes for analysis of HIF-1α and iNOS expressions. The results are representative examples of three similar experiments. Data are presented as the means S.E.M. and , compared to the sham-operated group (lane 1). Equal loading in each lane is demonstrated by similar intensities of -tubulin.