Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells
The role of JNK activation on ABE-mediated
apoptosis. THP-1 cells (2 × 105 cells ml−1) were treated
with 4 mg ml−1 of ABE for the indicated time. (a) Equal
amounts of whole cell lysates were resolved by SDS-PAGE, transferred to nitrocellulose
and probed with specific antibodies against p-JNK and JNK. (b) THP-1 cells
were pretreated with 10 mM NAC and then 4 mg ml−1 of ABE was treated up to 24 h.
Then, the cells were lysated and western blot was performed using anti-p-JNK and anti-JNK
antibodies. THP-1 cells were pretreated with SP600125 (20 M) for 2 h followed by ABE treatment
(4 mg ml−1) for 24 h. At the end of experiments, (c) DNA content and
(d) annexin-V were analyzed by flow cytometer. SP, SP600125.
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