Evidence-Based Complementary and Alternative Medicine / 2011 / Article / Fig 6

Original Article

Agaricus blazei Extract Induces Apoptosis through ROS-Dependent JNK Activation Involving the Mitochondrial Pathway and Suppression of Constitutive NF-κB in THP-1 Cells

Figure 6

Effect of ABE on constitutive NF-κB activation in THP-1 cells. THP-1 cells (2 × 105 cells ml−1) were incubated at 37°C with (a) the indicated time duration of 4 mg ml−1 of ABE and (b) the indicated concentrations at 12 h. DNA-binding activity of NF-κB was analyzed by chemiluminescent EMSA kit. (c) Western blots were performed for p65 and p50 protein levels in the nuclear extract. Nucleolin was used as an internal loading control. (d) Expression of IAP-1 and -2 was detected via western blot analysis. β-Actin was used as an internal loading control for cytoplasm. The results shown are from one representative experiment of three experiments that exhibited similar patterns.

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