Effect of Supplementation of Tanshinone IIA and Sodium Tanshinone IIA Sulfonate on the Anticancer Effect of Epirubicin: An In Vitro Study
Table 1
Interaction between STS (or Tan IIA) and epirubicin on the growth of BT-20 breast cancer cells.
Epirubicin (μg/mL)
STS (μg/mL)
Tan IIA (μg/mL)
Fa
CI
Effect
0.03125
25
0.048
2.014
Antagonistic
0.0625
25
0.0313
3.931
Antagonistic
0.125
25
0.0686
3.314
Antagonistic
0.25
25
0.0869
4.713
Antagonistic
0.5
25
0.1447
5.626
Antagonistic
1
25
0.2323
6.95
Antagonistic
2
25
0.7902
2.038
Antagonistic
0.03125
50
0.0492
3.166
Antagonistic
0.0625
50
0.0351
5.15
Antagonistic
0.125
50
0.0776
3.825
Antagonistic
0.25
50
0.0683
6.652
Antagonistic
0.5
50
0.2
4.557
Antagonistic
1
50
0.423
3.733
Antagonistic
2
50
0.8946
1.131
Additive/antagonistic
0.03125
25
0.6109
1.066
Additive/antagonistic
0.0625
25
0.5097
1.302
Additive/antagonistic
0.125
25
0.6354
1.197
Additive/antagonistic
0.25
25
0.8371
0.902
Synergistic
0.5
25
0.8257
1.141
Additive/antagonistic
1
25
0.8341
1.511
Antagonistic
2
25
0.8926
1.708
Antagonistic
0.03125
50
0.9412
1.013
Additive/antagonistic
0.0625
50
0.9422
1.018
Additive/antagonistic
0.125
50
0.9392
1.057
Additive/antagonistic
0.25
50
0.9382
1.106
Additive/antagonistic
0.5
50
0.9366
1.205
Additive/antagonistic
1
50
0.9372
1.377
Antagonistic
2
50
0.9374
1.728
Antagonistic
Fa: fraction affected; CI: combination index. A value of CI <1 represents a case where synergism of epirubicin and STS (or Tan IIA) was present. CI values of 1 and >1 represent additive and antagonistic effects, respectively.