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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 857124, 9 pages
http://dx.doi.org/10.1155/2011/857124
Research Article

Zataria multiflora Boiss and Carvacrol Affect β2-Adrenoceptors of Guinea Pig Trachea

1Department of Physiology and Pharmaceutical Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177948564, Iran
2Department of Biology, Faculty of Science, Islamic Azad University, Mashhad Branch, Mashhad 918714758, Iran

Received 16 May 2010; Revised 11 August 2010; Accepted 7 October 2010

Copyright © 2011 Mohammad Hossein Boskabady et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The stimulatory effect of Zataria multiflora Boiss (Labiatae) and carvacrol on β-adrenoceptors was examined on guinea pig trachea. The effects of three concentrations of aqueous-ethanolic extract, carvacrol, and propranolol (β-receptor antagonist) on β-adrenoceptors were tested in nonincubated (group 1, n=8) and incubated tracheal chains with 1 μM chlorpheniramine (histamine H1 receptor antagonist) (group 2, n=5). Isoprenaline (β-receptor agonist) curves obtained in the presence of all concentrations of the extract and carvacrol showed leftward shifts compared with that of saline in both groups. In both groups, the EC50 (the effective concentration of isoprenaline, causing 50% of maximum response) obtained in the presence of all concentrations of the extract and carvacrol was significantly lower compared to that of saline (P<.01 to P<.001). All values of (CR-1: (EC50 in the presence of active substances/EC50 obtained in the presence of saline)-1) obtained in the presence of concentrations of the extract and carvacrol in both groups were negative and significantly different from that of propranolol (P<.001 for all cases). The results indicated a stimulatory effect of Zataria multiflora Boiss extract on β2-adrenoceptors which is perhaps due to its constituent, carvacrol.