Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells
Figure 2
Induction of CYP3A4 activity and protein expression by 1α,25-(OH)2-D3. Caco-2 clone cells grown on culture inserts were treated with 1α,25-(OH)2-D3 (250 nM) for 3 weeks after confluence. (a) Metabolic activity of 1α,25-(OH)2-D3-treated Caco-2 clone cells. Nifedipine (200 M) was added to the apical compartment. After 4 hours, basolateral media was collected and analyzed for oxidized nifedipine. Results are means ± SD from triplicate experiments. **P < .01 versus control. (b) CYP3A4 expression level of 1α,25-(OH)2-D3-induced Caco-2 clone cells.
