Curdione Plays an Important Role in the Inhibitory Effect of Curcuma aromatica on CYP3A4 in Caco-2 Cells
Figure 5
Effects of MeOH extract, hexane fraction, curcumin and curdione on CYP3A4 protein and mRNA expression levels. Components were applied to the apical compartment and incubated for 72 hours. Then cell lysate or total RNA was prepared for western blot or real-time PCR. (a) Representative western immunoblot for CYP3A4 and quantitative analysis of CYP3A4 immunoprotein. The western immunoblot band intensities were normalized with that of GAPDH. Results are means ± SD from triplicate experiments. **P < .01 versus control. (b) Quantitative analysis of CYP3A4 mRNA. Results are means ± SD from triplicate experiments.
