A Preclinical Evaluation of Antrodia camphorata Alcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging

Figure 3

Induction of apoptosis in H441GL cells by ACAE treatment. (a) DNA agarose gel analysis. Genomic DNA samples were obtained from H441GL cells after incubation with different concentrations of ACAE. DNA fragmentation was observed in samples incubated with 100 and 150 μg mL^-1 of ACAE, whereas DNA samples from CGL1 cells remained intact. Approximately 5 μg of DNA samples were loaded in each lane. (b) Induction of apoptosis was analyzed by flow cytometry with anti-Annexin-V antibody. The intensity of Annexin-V signal in the H441GL cells increased as the concentration of ACAE increased. Quantitatively, the intensity of Annexin-V fluorescence detected in H441GL cells 48 h post-ACAE treatment was concentration dependent (lower panel, *P < .05; **P < .01). (c) Effect of ACAE on apoptotic pathways in H441GL cells. H441GL cells were treated with various concentrations of ACAE for 48 h. A concentration-dependent activation of both caspas-9 and its effector caspase-3 and the down-regulation of Bcl-2 were observed.