A Preclinical Evaluation of Antrodia camphorata Alcohol Extracts in the Treatment of Non-Small Cell Lung Cancer Using Non-Invasive Molecular Imaging
Figure 5
Non-invasive in vivo bioluminescence evaluation of ACAE-mediated anti-tumor effect. (a) In vitro bioluminescence examination of H441GL cells. H441GL cells demonstrated a substantially higher level of bioluminescence than control H441 cells. The insert depicts H441 control and H441GL cells under fluorescence microscope (GFP used for cell-sorting purpose). (b) ACAE treatment does not interfere with firefly luciferase activity in H441GL cells. (c) Control animals inoculated (s.c) with 105 H441GL cells, receiving vehicle treatment demonstrated a significantly higher tumor burden as assessed from the increasing intensity of the bioluminescent signals over the period of 28 days. Representative bioluminescence images of mice inoculated with 105 H441GL cells and received two different doses of ACAE oral gavages: 6.25 mg kg-1 3 days-1 and 31.25 mg kg-1 3 days-1, respectively (equivalent to 100 μg and 500 μg, resp.). Both treatment groups demonstrated a significant reduction in tumor burden when compared to the control group, especially in the mice treated with 500 μg of ACAE. (d) Scatter plots of superficial tumor size over time (measured by calliper) in response to two different concentrations of ACAE treatment.
