Original Article

8-Prenylkaempferol Suppresses Influenza A Virus-Induced RANTES Production in A549 Cells via Blocking PI3K-Mediated Transcriptional Activation of NF-κB and IRF3

Figure 6

Effect of wortmannin, LY294002 or 8-PK on PI3K activity in H1N1-infected A549 cells. For PIP3 production, cells were lysed at 16 h p.i. and then protein was extracted for immunoprecipitation and ELISA assay as described in Section 2. Data reported are mean SE of three independent experiments, each performed in triplicate. and , indicates significant differences as compared with H1N1 inoculation alone. Western blot analysis of the immunoprecipitates using anti-p85 antibodies confirmed that equal amounts of p85 protein were used in the assays (b).
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(a)
920828.fig.006b
(b)