Seed Oil of Brucea javanica Induces Apoptotic Death of Acute Myeloid Leukemia Cells via Both the Death Receptors and the Mitochondrial-Related Pathways
Effect of BJO on ROS release and mitochondrial membrane potential (MMP) collapse in U937 cells. U937 cells were treated with etoposide (10 μmol/L) or various concentrations of BJO as indicated for 6 h. (a) U937 cells were labeled with 5 μmol/L DCFH-DA for 1 h prior to the treatment with BJO. The addition of 100 μmol/L H2O2 for 1 h was used as a positive control for the H2O2 level. (b) Disruption of MMP was determined according to changes in fluorescence density using rhodamine 123. (c, d, and e). ROS accumulation, MMP, and cell cycle examination of U937 pretreated with antioxidants. Cells were pretreated with catalase (500 U/mL) or N-acetylcysteine (10 mM) for 4 hours, followed by treatment with or without BJO for another 6 h. ROS level, MMP, and cell cycle were measured using flow cytometry as described in Materials and Methods.
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