Evidence-Based Complementary and Alternative Medicine / 2011 / Article / Fig 5

Research Article

Seed Oil of Brucea javanica Induces Apoptotic Death of Acute Myeloid Leukemia Cells via Both the Death Receptors and the Mitochondrial-Related Pathways

Figure 5

Effect of BJO on human peripheral blood lymphocytes and AML cells. AML cells and PBLs were treated with emulsifier (negative control), etoposide (10 μmol/L, positive control), or indicated concentrations of BJO for 6 hours. (a) Cell viabilities were determined by MTT assay. *** (b) Apoptotic cells were evaluated by cell cycle analysis. Percentage of cells in the subG1 phase was measured using FACS as described in Materials and Method. (c) Disruptions of MMP were determined by changes in fluorescence density using rhodamine 123.
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