HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells
Figure 2
Inhibitory effects of HMC05 and berberine on TNF-α-induced mRNA expression of MCP-1, VCAM-1 and ICAM-1 in HUVECs. Serum-starved HUVECs were treated with HMC05 and berberine in the absence ((a) and (b)) or presence (c–e) of TNF-α (10 ng ml−1). After 3 h, the cells were harvested, and the mRNA expression of MCP-1 ((a) and (c)), VCAM-1 ((b) and (d)) and ICAM-1 (e) was quantified by real-time RT-PCR. Data are expressed as the mean ± SEM of four independent experiments with duplicates. *P < .05 compared with untreated control group. #P < .05 compared with TNF-α-treated group.