Original Article

HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells

Figure 5

HMC05 suppresses TNF-α-induced geranylgeranyl isoprenoid-dependent ROS generation. HUVECs were treated with TNF-α in the presence or absence of HMC05 and/or GGPP for 1 h, and incubated with DCF-DA (5 μM) for an additional 5 min. The intensity of DCF fluorescence detecting intracellular ROS was analyzed by using ImageInside program. Data are expressed as the mean ± SEM of three independent experiments. *P < .01 compared with untreated control group. #P < .01 compared with the TNF-α-treated group. $P < .01 compared with TNF-α and HMC05-treated group.
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