Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells

Figure 11

Effects of EAS5 on the protein levels of cyclin B1 and p21 in NB4 cells. EAS5 down-regulated cyclin B1 and elevated p21 protein levels in NB4 cells. The cells were treated with PBS or EAS5 (3.75–30 μg/ml) for 72 h and then harvested. Cell lysates were then prepared for western blot analysis. EAS5: active fraction of THL.