Research Article

Anti-Inflammatory Activity Is a Possible Mechanism by Which the Polyherbal Formulation Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Root), and Smilax glabra (Rhizome) Mediates Its Antihepatocarcinogenic Effects

Figure 4

Immunohistochemical evaluation of NF-κB nuclear translocation in hepatocytes of C3H mice injected with DEN and subsequently treated with the decoction. Liver sections were incubated with primary antibody to the p 65 subunit of NF-κB as described in the methods. (a) After intraperitoneal injections of DEN (×100), (b) healthy mice (×40), (c) treated with the decoction after intraperitoneal injection of DEN (×100), and (d) negative control; section stained without primary antibody (×100).
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