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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 125945, 14 pages
Research Article

Development of a Biocrystallisation Assay for Examining Effects of Homeopathic Preparations Using Cress Seedlings

1Institute of Complementary Medicine KIKOM, University of Bern, Insel-Spital, 3010 Bern, Switzerland
2Hiscia Institute, Kirschweg 9, 4144 Arlesheim, Switzerland
3Center for Integrative Medicine, University of Witten/Herdecke, Gerhard-Kienle-Weg 4, 58313 Herdecke, Germany
4Louis Bolk Institute, Hoofdstraat 24, 3972 LA Driebergen, The Netherlands
5Crystal Lab, Landgoed Roepaen, Kleefseweg 9, 6595 NK Ottersum, The Netherlands
6Biodynamic Research Association Denmark, Landsbyvaenget 7, Herskind, 8464 Galten, Denmark

Received 13 December 2011; Accepted 17 April 2012

Academic Editor: Thomas Ostermann

Copyright © 2012 S. Baumgartner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A major challenge in basic research into homeopathic potentisation is to develop bioassays that yield consistent results. We evaluated the potential of a seedling-biocrystallisation method. Cress seeds (Lepidium sativum L.) germinated and grew for 4 days in vitro in Stannum metallicum 30x or water 30x in blinded and randomized assignment. 15 experiments were performed at two laboratories. CuCl2-biocrystallisation of seedlings extracted in the homeopathic preparations was performed on circular glass plates. Resulting biocrystallograms were analysed by computerized textural image analysis. All texture analysis variables analysed yielded significant results for the homeopathic treatment; thus the texture of the biocrystallograms of homeopathically treated cress exhibited specific characteristics. Two texture analysis variables yielded differences between the internal replicates, most probably due to a processing order effect. There were only minor differences between the results of the two laboratories. The biocrystallisation method seems to be a promising complementary outcome measure for plant bioassays investigating effects of homeopathic preparations.