Antioxidant, Antinociceptive, and Anti-Inflammatory Activities from Actinidia callosa var. callosa In Vitro and In Vivo
Figure 2
The effects of the methanolic extract and fractions from the stem of A. callosa var. callosa on lipopolysaccharide-(LPS-)induced cell viability (a) and NO production (b) of RAW 264.7 macrophages. Cells were incubated for 24 h with 100 ng/mL of LPS in the absence or presence of samples (0, 125, 250, 500, and 1000 μg/mL). Cell viability assay was performed using MTT assay. Nitrite concentration in the medium was determined using Griess reagent. M: methanol extract; H: n-hexane fraction; EA: ethyl-acetate fraction; B: n-butanol fraction; W: water fraction; The data were presented as mean ± S.D. for three different experiments performed in triplicate. ###Compared with sample of control group. *, **, and *** were compared with LPS-alone group.
