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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 135379, 10 pages
Research Article

Analgesic and Anti-Inflammatory Activities of Methanol Extract of Cissus repens in Mice

1Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung 404, Taiwan
2Department of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan
3Department of Biotechnology, TransWorld University, 1221 Zhennan Rd., Douliu City, Yunlin County 640, Taiwan

Received 1 June 2012; Revised 25 July 2012; Accepted 5 August 2012

Academic Editor: Jairo Kenupp Bastos

Copyright © 2012 Ching-Wen Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study was to investigate possible analgesic and anti-inflammatory mechanisms of the C R M e O H . Analgesic effect was evaluated in two models including acetic acid-induced writhing response and formalin-induced paw licking. The anti-inflammatory effect was evaluated by λ-carrageenan-induced mouse paw edema and histopathologic analyses. The results showed that C R M e O H (500 mg/kg) decreased writhing response in the acetic acid assay and licking time in the formalin test. C R M e O H (100 and 500 mg/kg) significantly decreased edema paw volume at 4th to 5th hours after λ-carrageenan had been injected. Histopathologically, C R M e O H abated the level of tissue destruction and swelling of the edema paws. These results were indicated that anti-inflammatory mechanism of C R M e O H may be due to declined levels of NO and MDA in the edema paw through increasing the activities of SOD, GPx, and GRd in the liver. Additionally, C R M e O H also decreased IL-1β, IL-6, NFκB, TNF-α, COX-2, and iNOS levels. The contents of two active ingredients, ursolic acid and lupeol, were quantitatively determined. This paper demonstrated possible mechanisms for the analgesic and anti-inflammatory effects of C R M e O H and provided evidence for the classical treatment of Cissus repens in inflammatory diseases.