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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 156563, 10 pages
http://dx.doi.org/10.1155/2012/156563
Research Article

Effects of Vitamin D Supplementation during the Induction and Progression of Osteoarthritis in a Rat Model

1Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), IPN, Avenida IPN No. 2508, 07360 México, DF, Mexico
2Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), IPN, Avenida IPN No. 2508, 07360 México, DF, Mexico
3Unidad de Investigación en Infectología e Inmunología, Hospital de Infectología Dr. Daniel Méndez Hernández, Centro Médico Nacional La Raza, IMSS, Avenida Jacarandas s/n, 02990 México, DF, Mexico
4Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Circuito Escolar s/n, 04510 México, DF, Mexico

Received 18 April 2012; Revised 4 September 2012; Accepted 5 September 2012

Academic Editor: Angelo Antonio Izzo

Copyright © 2012 E. C. Castillo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Epidemiological studies correlate low levels of vitamin D with the osteoarthritis (OA) progression. Cytokines and metalloproteases play a major role in OA promoting the inflammation and degradation of the cartilage and can be induced through the Toll-like receptor (TLR) pathway. The aim of this study was to evaluate the protective effect of vitamin D supplementation on the development of osteoarthritis (OA) through examining the genetic regulation of TLRs, cytokines, and metalloproteases in chondrocytes as well as the wideness of cartilage in rats with OA. Our results demonstrate that the signaling through TLR-4 is a proinflammatory mechanism in osteoarthritis that drives the upregulation of MMP-3, IL-1β, and TNF-α gene expression, leading to cartilage degradation and inflammation. Vitamin D supplementation had a protective effect during the onset but not during the chronic stage of OA in the rat model.