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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 156563, 10 pages
http://dx.doi.org/10.1155/2012/156563
Research Article

Effects of Vitamin D Supplementation during the Induction and Progression of Osteoarthritis in a Rat Model

1Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), IPN, Avenida IPN No. 2508, 07360 México, DF, Mexico
2Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), IPN, Avenida IPN No. 2508, 07360 México, DF, Mexico
3Unidad de Investigación en Infectología e Inmunología, Hospital de Infectología Dr. Daniel Méndez Hernández, Centro Médico Nacional La Raza, IMSS, Avenida Jacarandas s/n, 02990 México, DF, Mexico
4Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Circuito Escolar s/n, 04510 México, DF, Mexico

Received 18 April 2012; Revised 4 September 2012; Accepted 5 September 2012

Academic Editor: Angelo Antonio Izzo

Copyright © 2012 E. C. Castillo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

RNA was isolated from chondrocytes of knee cartilage samples and analyzed for the expression of TLR-4, IL-1β, TNF-α, and MMP-3 by a Real Time PCR system (Mod. 7500, Applied Biosystems, Carlsbad, CA, USA). The amounts of specific mRNA in the samples were calculated by the ΔΔCT method and the results were expressed as the relative expression level data (2-ΔΔCт). A correlation between the levels of relative expression of TLR-4 and IL-1β, TNF-α, and MMP-3 was performed using Pearson’s correlation (Supplementary Figure 1).

4 IU (100 ng/kg/day) of vitamin D were administrated orally 3 days before surgery, and daily administrated until the last day of OA induction and/or progression (Figure 1). For the evaluation of vitamin D supplementation during OA progression, three experimental subgroups were included: (1) rats without vitamin supplementation during HIE + progression without vitamin supplementation (nV+PnV), (2) rats supplemented with the vitamin during HIE + progression without vitamin supplementation of vitamin (sV+PnV) and (3) rats supplemented with the vitamin during HIE + progression with vitamin supplementation (sV+PsV). The hypertrophy was evaluated measuring the wideness of the condyles using images from a stereoscopy microscope (Leica EZ4D. Bensheim, Alemania) and the software Gimp (GNU Image Manipulation Program). (Supplementary Figure 2).

  1. Supplementary Figures