TY - JOUR
A2 - Selvan, Senthamil R.
AU - Saha, S.
AU - Mukhopadhyay, M. K.
AU - Ghosh, P. D.
AU - Nath, D.
PY - 2012
DA - 2012/09/05
TI - Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice
SP - 176385
VL - 2012
AB - The aim of the present study was to investigate the protective role of methanolic leaf extract of Ocimum basilicum L. against benzene-induced hematotoxicity in Swiss albino mice. GC analysis and subacute toxicity level of the extract were tested. Mice were randomly divided into three groups among which II and III were exposed to benzene vapour at a dose 300 ppm × 6 hr/day × 5 days/week for 2 weeks and group I was control. Group III of this experiment was treated with the leaf methanolic extract at a dose of 100 mg/kg body weight, a dose in nontoxic range. Hematological parameters (Hb%, RBC and WBC counts), cell cycle regulatory proteins expression and DNA fragmentation analysis of bone marrow cells was performed. There was an upregulation of p53 and p21 and downregulation of levels of CDK2, CDK4, CDK6, and cyclins D1 and E in leaf extract-treated group. DNA was less fragmented in group III compared to group II (P<0.05). The present study indicates that the secondary metabolites of O. basilicum L. methanolic leaf extract, comprising essential oil monoterpene geraniol and its oxidized form citral as major constituents, have modulatory effect in cell cycle deregulation and hematological abnormalities induced by benzene in mice.
SN - 1741-427X
UR - https://doi.org/10.1155/2012/176385
DO - 10.1155/2012/176385
JF - Evidence-Based Complementary and Alternative Medicine
PB - Hindawi Publishing Corporation
KW -
ER -