Table 1: Amino acid composition (mg/g dry weight) of S. horrens hydrolysates after 5 h hydrolysis using different proteasesa.

Amino acidSea cucumberAlcalaseFlavourzymeTrypsinPapainBromelainProtamex

Asp22.87 ± 1.00c32.52 ± 3.29ab36.35 ± 4.76a30.23 ± 0.14b10.76 ± 0.02e17.84 ± 0.57d23.60 ± 0.15c
Glu53.85 ± 0.72b56.93 ± 0.00b76.97 ± 4.86a56.34 ± 0.00b24.92 ± 0.02e35.13 ± 1.01d47.36 ± 0.00c
Ser16.00 ± 1.36b15.32 ± 0.65bc20.04 ± 0.20a15.65 ± 0.21b8.13 ± 0.01e11.72 ± 0.46d13.92 ± 0.06c
Gly66.21 ± 1.37b61.57 ± 1.00c88.64 ± 2.58a61.18 ± 0.42c20.78 ± 0.26e21.62 ± 0.93e54.76 ± 0.53d
Arg35.98 ± 0.49b32.98 ± 0.57c40.02 ± 0.72a33.33 ± 0.14c11.42 ± 0.19f14.95 ± 0.00e23.89 ± 0.12d
Thr10.36 ± 0.19d9.62 ± 0.15d23.64 ± 0.54a9.74 ± 0.42d9.58 ± 0.06d13.62 ± 0.75c15.96 ± 0.61b
Ala52.59 ± 0.13a49.42 ± 0.54b41.16 ± 0.85c49.15 ± 0.21 b9.48 ± 0.18f12.40 ± 0.64e24.04 ± 0.60d
Pro48.27 ± 0.13a44.89 ± 0.14b40.09 ± 0.94c43.45 ± 0.35b11.12 ± 0.16f13.44 ± 0.63e25.40 ± 0.78d
Tyr9.29 ± 0.35b8.14 ± 0.02c10.34 ± 0.20a7.59 ± 0.02d4.51 ± 0.02f6.87 ± 0.05e7.24 ± 0.18de
Val16.81 ± 0.90a14.01 ± 0.16b16.92 ± 0.10a13.78 ± 0.04bc7.53 ± 0.01d12.24 ± 0.38bc12.00 ± 0.08c
Ile17.21 ± 0.18a15.13 ± 0.11b16.95 ± 0.17a14.66 ± 0.07b7.98 ± 0.10d12.95 ± 0.59c12.96 ± 0.21c
Leu15.53 ± 0.22b13.96 ± 0.16c16.70 ± 0.38a13.82 ± 0.01c7.06 ± 0.10f12.24 ± 0.50d10.75 ± 0.13e
Phe7.22 ± 0.14b6.44 ± 0.03c7.83 ± 0.52a6.15 ± 0.02c4.23 ± 0.01d7.47 ± 0.40ab6.01 ± 0.07c
Lys11.48 ± 0.04b10.01 ± 0.10d13.48 ± 0.12a10.75 ± 0.02c7.52 ± 0.06f11.60 ± 0.56b9.32 ± 0.06a
Hydrophobic AA281.47258.51278.78253.2883.86112.72153.20
Hydrophilic AA154.66160.91215.30159.8774.19107.75100.87
positively charged AA47.4743.0053.5144.0918.9526.5633.21

aData are means ± SD of triplicate determinations. Means with different superscript letters within the same row indicate significant differences among hydrolysates ( ).