Evidence-Based Complementary and Alternative Medicine

Research Article

Enzyme Hydrolysates from Stichopus horrens as a New Source for Angiotensin-Converting Enzyme Inhibitory Peptides

Table 1

Amino acid composition (mg/g dry weight) of S. horrens hydrolysates after 5 h hydrolysis using different proteases^a.


Amino acid	Sea cucumber	Alcalase	Flavourzyme	Trypsin	Papain	Bromelain	Protamex

Asp	22.87 ± 1.00^c	32.52 ± 3.29^ab	36.35 ± 4.76^a	30.23 ± 0.14^b	10.76 ± 0.02^e	17.84 ± 0.57^d	23.60 ± 0.15^c
Glu	53.85 ± 0.72^b	56.93 ± 0.00^b	76.97 ± 4.86^a	56.34 ± 0.00^b	24.92 ± 0.02^e	35.13 ± 1.01^d	47.36 ± 0.00^c
Ser	16.00 ± 1.36^b	15.32 ± 0.65^bc	20.04 ± 0.20^a	15.65 ± 0.21^b	8.13 ± 0.01^e	11.72 ± 0.46^d	13.92 ± 0.06^c
Gly	66.21 ± 1.37^b	61.57 ± 1.00^c	88.64 ± 2.58^a	61.18 ± 0.42^c	20.78 ± 0.26^e	21.62 ± 0.93^e	54.76 ± 0.53^d
Arg	35.98 ± 0.49^b	32.98 ± 0.57^c	40.02 ± 0.72^a	33.33 ± 0.14^c	11.42 ± 0.19^f	14.95 ± 0.00^e	23.89 ± 0.12^d
Thr	10.36 ± 0.19^d	9.62 ± 0.15^d	23.64 ± 0.54^a	9.74 ± 0.42^d	9.58 ± 0.06^d	13.62 ± 0.75^c	15.96 ± 0.61^b
Ala	52.59 ± 0.13^a	49.42 ± 0.54^b	41.16 ± 0.85^c	49.15 ± 0.21^b	9.48 ± 0.18^f	12.40 ± 0.64^e	24.04 ± 0.60^d
Pro	48.27 ± 0.13^a	44.89 ± 0.14^b	40.09 ± 0.94^c	43.45 ± 0.35^b	11.12 ± 0.16^f	13.44 ± 0.63^e	25.40 ± 0.78^d
Tyr	9.29 ± 0.35^b	8.14 ± 0.02^c	10.34 ± 0.20^a	7.59 ± 0.02^d	4.51 ± 0.02^f	6.87 ± 0.05^e	7.24 ± 0.18^de
Val	16.81 ± 0.90^a	14.01 ± 0.16^b	16.92 ± 0.10^a	13.78 ± 0.04^bc	7.53 ± 0.01^d	12.24 ± 0.38^bc	12.00 ± 0.08^c
Ile	17.21 ± 0.18^a	15.13 ± 0.11^b	16.95 ± 0.17^a	14.66 ± 0.07^b	7.98 ± 0.10^d	12.95 ± 0.59^c	12.96 ± 0.21^c
Leu	15.53 ± 0.22^b	13.96 ± 0.16^c	16.70 ± 0.38^a	13.82 ± 0.01^c	7.06 ± 0.10^f	12.24 ± 0.50^d	10.75 ± 0.13^e
Phe	7.22 ± 0.14^b	6.44 ± 0.03^c	7.83 ± 0.52^a	6.15 ± 0.02^c	4.23 ± 0.01^d	7.47 ± 0.40^ab	6.01 ± 0.07^c
Lys	11.48 ± 0.04^b	10.01 ± 0.10^d	13.48 ± 0.12^a	10.75 ± 0.02^c	7.52 ± 0.06^f	11.60 ± 0.56^b	9.32 ± 0.06^a
Hydrophobic AA	281.47	258.51	278.78	253.28	83.86	112.72	153.20
Hydrophilic AA	154.66	160.91	215.30	159.87	74.19	107.75	100.87
positively charged AA	47.47	43.00	53.51	44.09	18.95	26.56	33.21

^aData are means ± SD of triplicate determinations. Means with different superscript letters within the same row indicate significant differences among hydrolysates ().