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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 237482, 8 pages
http://dx.doi.org/10.1155/2012/237482
Research Article

Cyprinus carpio Decoction Improves Nutrition and Immunity and Reduces Proteinuria through Nephrin and CD2AP Expressions in Rats with Adriamycin-Induced Nephropathy

1Department of Nutrition, Tianjin Third Central Hospital, Tianjin 300170, China
2Tianjin Key Laboratory of Artificial Cells, Tianjin Third Central Hospital, Tianjin 300170, China
3Department of Nutrition, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300000, China
4Institute of Integrative medicine, Qingdao University Medical College, Qingdao 266021, China
5Department of Nutrition, The First Affiliated Hospital of Soochow University, Jiangsu, Suzhou 215000, China

Received 29 November 2011; Revised 3 March 2012; Accepted 19 March 2012

Academic Editor: Cheorl-Ho Kim

Copyright © 2012 Yumei Qi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cyprinus carpio decoction (CCD) is a well-known Chinese food medicine formula, accepted widely as a useful therapy in preventing edema and proteinuria caused by renal disease. However, the mechanism underlying this effect remains unclear. The current study investigated the potential mechanism of CCD in alleviating nephropathy induced by adriamycin (ADR) in rats. 70  eight-week-old Wistar rats were randomly divided into normal, model, fosinopril, YD, YG groups. All rats except for the normal group received 6.5 mg/kg⋅bw of ADR injection into the vena caudalis once. Different doses of CCD (11.3 and 22.5 g kg-1) were lavaged to rats in YD and YG groups, respectively. Then the serum biochemical values of the total protein (TP), albumin (ALB), blood urea nitrogen (BUN), creatinine (Cr), electrolyte levels, and the urinary protein (UP) content in 12 hr urine were measured. Interleukin-4 (IL-4) and interferon (INF-γ) were measured by enzyme-like immunosorbent assay (ELISA). The pathomorphological analysis was observed using light and electron microscopy, and the expressions of nephrin and CD2-associated protein (CD2AP) in renal tissues were determined by immunohistochemical assay. The results indicated that CCD can relieve ADR-induced nephropathy (ADN) by improving the nutrition status, regulating the immunity, and inhibiting proteinuria by increasing nephrin and CD2AP expressions.