Sesamin: A Naturally Occurring Lignan Inhibits CYP3A4 by Antagonizing the Pregnane X Receptor Activation
Figure 8
Disruption of protein-protein interactions between PXR and SRC-1/HNF4α in the presence of sesamin in a mammalian two-hybrid assay. Mammalian two-hybrid studies were conducted in LS174T cells to elucidate the effects of sesamin on the interactions between (a) SRC-1 receptor-interacting domain (RID) (pBIND-SRC-1); (b) full-length HNF4α (pACT-HNF4α), and the full-length clone of PXR (pACT-PXR and pBIND-PXR, resp.). The schematic diagram of the plasmids used is illustrated at the left top of each figure. The cells were harvested in equal aliquots at 24 h to determine luciferase activity. All transfections were normalized for transfection efficiency by β-galactosidase activity. The results are expressed as mean ± SD (); *; **; and ***, as compared to DMSO-treated or 20 μM rifampin-treated cells, as indicated.
