Sesamin: A Naturally Occurring Lignan Inhibits CYP3A4 by Antagonizing the Pregnane X Receptor Activation

Table 1

Primer sequences used for real-time polymerase chain reaction.

Gene		Primer sequence (5′–3′)	PCR condition
CYP3A4	(F)	5′-GGG AAG CAG AGA CAG GCA AG-3′	Denaturation (30 s at 95°C)
	(R)	5′-GAG CGT TTC ATT CAC CAC CA-3′
UGT1A1	(F)	5′-TTT TGT CTG GCT GTT CCC ACT-3′	Annealing (30 s at 60°C)
	(R)	5′-GAA GGT CAT GT GAT CTG AAT GAG A-3′
NQO1	(F)	5′-CCA TTC TGA AAG GCT GGT TTG-3′	Extension (30 s at 72°C)
	(R)	5′-CTA GCT TTG ATC TGG TTG TC-3′
β-actin	(F)	5′-GTG GGG CGC CCC AGG CAC CA-3′	45 cycles
	(R)	5′-CAC CCC GCG GGG TCC GTG GT-3′

(F): forward primer; (R): reverse primer.