Cordyceps militaris Grown on Germinated Soybean Induces G2/M Cell Cycle Arrest through Downregulation of Cyclin B1 and Cdc25c in Human Colon Cancer HT-29 Cells
Figure 1
Anti-proliferative effects of GSC, GS, and CM BuOH extract on HT-29 cells. (a) HT-29 cells (1 × 104/well) were treated with various concentrations (0, 25, 50, 75, 100, and 250 μg/mL) of GSC, GS, CM BuOH, extract and 25 μg/mL of doxorubicin for 48 h. Doxorubicin, a potent anticancer agent, was used as the positive control. The HT-29 cell proliferation was determined by the EZ-CyTox assay. One-way ANOVA followed by Dunnett’s t-test was used for comparisons of multiple group means (a or b versus blank). (b) Effect of GSC BuOH extract on HT-29 cell proliferation. Time-dependent inhibition of HT-29 cell proliferation by the GSC BuOH extracts. The growth inhibition was calculated as a percentage of inhibition and compared with the values for the control. One-way ANOVA followed by Dunnett’s t-test was used for comparisons of multiple group means (* versus control (48 h), # or ## versus control (72 h), * control (48 h) versus control (72 h)). GSC, Cordyceps militaris grown on germinated soybean; GS, germinated soybean; CM, Cordyceps militaris.
