254960.fig.004a
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254960.fig.004b
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Figure 4: Effects of SLE and diosgenin on WEHI-3 cells in the extrinsic apoptotic pathway. Cells were pretreated with specific inhibitors of caspases-8 (z-IETD-fmk) for 1 h after exposure to SLE (200 μg/mL) or diosgenin (50 μM) for 12, 24, 36, and 48 h. (a) The whole-cell lysates were subjected to caspase-8 activity and (b) cells were collected after SLE or diosgenin for a 48 h treatment to determine the percentage of viable cells. (c) Cells were incubated with 200 μg/mL of SLE or 50 μM of diosgenin for 24 h, and FasL protein expression was detected by immunostaining and analysis by flow cytometry. (d) Cells were pretreated with anti-FasL mAb or specific inhibitor of p53 (PFTα) for 1 h after exposure to SLE (200 μg/mL) or diosgenin (50 μM) for a 48 h exposure. Cells were collected to determine the percentage of viable cells. Data are presented as the mean ± S.E.M. of three independent experiments. *, , significantly different compared with SLE-treated cells.